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KMID : 0379519990150030353
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한국독성학회지
1999 Volume.15 No. 3 p.353 ~ p.361
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Biological Function of the Extracts from Ficus Carica Linnaeus (FCL)
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Lee Jeong-Chae
Jung Hee-Young
Lim Kye-Taek
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Abstract
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To measure antioxidative activities, the ethanol or water extracts from Ficus carica Linnaeus (FCL) were tested with ammonium thiocyanate, deoxyribose, and hypoxanthine/xanthine oxidase assays. Also, this study was carried out to investigate the effects of FCL extracts on cholesterol level in mouse plasma and on apoptosis induced by 12-O-tetradecanoylphorbol 13-acetate (TPA) in mouse thymocytes, with dividing four samples (water or ethanol extracts from FCL stem or leaf). In thiocyanate assay, the antioxidative activities of the ethanol extracts were shown stronger in stem extract than that in leaf extract. In deoxyribose and hypoxanthine/xanthine oxidase assay, all FCL extracts resulted to scasuperoxide (O2) as well as hydroxyl radical (OH) efficiently. Especially the antioxidative activities of FCL extracts were shown more specific to superoxide than hydroxyl radical. To know the change of cholesterol level in plasma, extracts from FCL stem administrated orally to ICR mice with 600 mg/kg once a day for 10 days. In general, the results showed that extracts from FCL stem strongly reduced cholesterol level in plasma. Particularly, the concentrations of triglyceride (TG), and very low density lipoprotein (VLDL) in transformation of cholesterols were detected significantly lower (p<0.001) in treatment with extracts from FCL stem, compared to that of control excepted administration of the FCL extracts. In the level of low density lipoprotein (LDL), water extract of FCL stem lowered significantly (p<0.05), while ethanol extract did not so. 1n agarose gel electrophoresis to investigate anti-apoptotic effect of FCL extracts in mouse thymocytes, DNA fragmentation induced by 100 nM TPA was reduced in a dose-dependent manner in treatment of FCL extracts. Conclusively, this experiment showed that FCL extracts have an antioxidaactivity and reduced cholesterol level in mouse plasma. Also these results suggest that TPA induce apoptosis in thymocytes, and that FCL extracts can inhibit TPA-induced DNA fragmentation of ~200 base pairs having biochemical nature of programmed cell death in immune cell.
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KEYWORD
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Ficus carica Linnaeus (FCL), Antioxidant, Plasma cholesterols, 12-O-tetradecanoylphor-bol 13-acetate (TPA), Apoptosis, Mouse thymocytes
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